Integrative analysis reveals novel associations between DNA methylation and the serum metabolome of adolescents with type 2 diabetes: A cross-sectional study.
Abstract
Objective: Rates of type 2 diabetes (T2D) among adolescents are on the rise. Epigenetic changes could be associated with the metabolic alterations in adolescents with T2D.
Methods: We performed a cross sectional integrated analysis of DNA methylation data from peripheral blood mononuclear cells with serum metabolomic data from First Nation adolescents with T2D and controls participating in the Improving Renal Complications in Adolescents with type 2 diabetes through Research (iCARE) cohort study, to explore the molecular changes in adolescents with T2D.
Results: Our analysis showed that 43 serum metabolites and 36 differentially methylated regions (DMR) were associated with T2D. Several DMRs were located near the transcriptional start site of genes with established roles in metabolic disease and associated with altered serum metabolites (e.g. glucose, leucine, and gamma-glutamylisoleucine). These included the free fatty acid receptor-1 (FFAR1), upstream transcription factor-2 (USF2), and tumor necrosis factor-related protein-9 (C1QTNF9), among others.
Conclusions: We identified DMRs and metabolites that merit further investigation to determine their significance in controlling gene expression and metabolism which could define T2D risk in adolescents.
Citations
Altmetric:
Date
2022-10-10
Type
Article
Subject
Pharmacology, Paediatrics, Biochemistry, Oncology. Pathology., Microbiology. Immunology
Collections
Citation
Agarwal P, Wicklow BA, Dart AB, Hizon NA, Sellers EAC, McGavock JM, Talbot CPJ, Fonseca MA, Xu W, Davie JR, Jones MJ, Acharjee A, Dolinsky VW. Integrative analysis reveals novel associations between DNA methylation and the serum metabolome of adolescents with type 2 diabetes: A cross-sectional study. Front Endocrinol (Lausanne). 2022 Oct 10;13:934706. doi: 10.3389/fendo.2022.934706
Journal / Source Title
Frontiers in Endocrinology
DOI
10.3389/fendo.2022.934706
PMID
36303872
Publisher
Frontiers Media
Publisher’s URL
https://www.frontiersin.org/journals/endocrinology
https://www.ncbi.nlm.nih.gov/pmc/journals/1753/
https://www.ncbi.nlm.nih.gov/pmc/journals/1753/