Crosstalk between AML and stromal cells triggers acetate secretion through the metabolic rewiring of stromal cells
Abstract
Acute myeloid leukaemia (AML) cells interact and modulate components of their surrounding microenvironment into their own benefit. Stromal cells have been shown to support AML survival and progression through various mechanisms. Nonetheless, whether AML cells could establish beneficial metabolic interactions with stromal cells is underexplored. By using a combination of human AML cell lines and AML patient samples together with mouse stromal cells and a MLL-AF9 mouse model, here we identify a novel metabolic crosstalk between AML and stromal cells where AML cells prompt stromal cells to secrete acetate for their own consumption to feed the tricarboxylic acid cycle (TCA) and lipid biosynthesis. By performing transcriptome analysis and tracer-based metabolic NMR analysis, we observe that stromal cells present a higher rate of glycolysis when co-cultured with AML cells. We also find that acetate in stromal cells is derived from pyruvate via chemical conversion under the influence of reactive oxygen species (ROS) following ROS transfer from AML to stromal cells via gap junctions. Overall, we present a unique metabolic communication between AML and stromal cells and propose two different molecular targets, ACSS2 and gap junctions, that could potentially be exploited for adjuvant therapy.
Author
Vilaplana-Lopera, Nuria
Cuminetti, Vincent
Almaghrabi, Ruba
Papatzikas, Grigorios
Rout, Ashok Kumar
Jeeves, Mark
González, Elena
Alyahyawi, Yara
Cunningham, Alan
Erdem, Ayşegül
Schnütgen, Frank
Raghavan, Manoj
Potluri, Sandeep
Cazier, Jean-Baptiste
Schuringa, Jan Jacob
Reed, Michelle A C
Arranz, Lorena
Günther, Ulrich L
Garcia, Paloma
Raghavan, Manoj
Cuminetti, Vincent
Almaghrabi, Ruba
Papatzikas, Grigorios
Rout, Ashok Kumar
Jeeves, Mark
González, Elena
Alyahyawi, Yara
Cunningham, Alan
Erdem, Ayşegül
Schnütgen, Frank
Raghavan, Manoj
Potluri, Sandeep
Cazier, Jean-Baptiste
Schuringa, Jan Jacob
Reed, Michelle A C
Arranz, Lorena
Günther, Ulrich L
Garcia, Paloma
Raghavan, Manoj
Citations
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Date
2022-09-02
Type
Article
Subject
Oncology. Pathology., Biochemistry, Clinical pathology
Collections
Citation
Vilaplana-Lopera N, Cuminetti V, Almaghrabi R, Papatzikas G, Rout AK, Jeeves M, González E, Alyahyawi Y, Cunningham A, Erdem A, Schnütgen F, Raghavan M, Potluri S, Cazier JB, Schuringa JJ, Reed MAC, Arranz L, Günther UL, Garcia P. Crosstalk between AML and stromal cells triggers acetate secretion through the metabolic rewiring of stromal cells. Elife. 2022 Sep 2;11:e75908. doi: 10.7554/eLife.75908.
Journal / Source Title
eLife
DOI
10.7554/eLife.75908
PMID
36052997
Publisher
eLife Sciences Publications
Publisher’s URL
https://elifesciences.org/articles/75908