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pMTL60000: A modular plasmid vector series for parageobacillus thermoglucosidasius strain engineering
Minton, Nigel P.
Minton, Nigel P.
Abstract
Parageobacillus thermoglucosidasius is a promising chassis for producing chemicals and fuels. Here we designed, built and tested the pMTL60000 modular plasmids containing standardised Gram-positive and Gram-negative replicons, selectable markers and application-specific modules. The pMTL60000 modular plasmids were characterised with regard to transformation efficiency, segregational stability, copy number and compatibility.Copyright © 2022 The Authors
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Date
2022
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Madika, A., Spencer, J., Lau, M.S.H., Sheng, L., Zhang, Y. and Minton, N.P. (2022) 'pMTL60000: A modular plasmid vector series for parageobacillus thermoglucosidasius strain engineering', Journal of Microbiological Methods, 202, pp. 106600. doi: 10.1016/j.mimet.2022.106600 https://doi.org/10.1016/j.mimet.2022.106600.